Pathways & Frameworks

The biological circuits behind every patient — visualized. Each diagram shows the steps, the cofactors, the genes that fail, and the interventions that rescue.

Ion-Channel Gates — ‘Treat the gates first’

Reed/Dickerson’s framework. An action potential opens the sodium gate (ANK3); sodium influx triggers the calcium gate (CACNA1C); calcium is the master key that releases all neurotransmitters. Toggle Variant gates to see what mood instability looks like — then toggle Lithium to stabilize.

Watch the calcium master-key open the vesicles.
ANK3 Na⁺ gate CACNA1C Ca²⁺ gate vesicles 5-HT · DA · NE GABA · melatonin Li⁺ Li⁺ irregular firing over-active gate erratic NT release

Dickerson & Reed 2023 (JAANP) — low-dose lithium in CACNA1C-A-allele carriers: ~66% reduction in PHQ-9, ~65% reduction in GAD-7, both p<.001. Reed: ‘Lithium is the cake. Everything else is the icing.’

Methylation Cycle

Folate + B12 + B6 (P5P) + B2 → methyl groups for DNA, neurotransmitters, homocysteine clearance. Toggle MTHFR C677T T/T to see the throughput collapse — then toggle L-methylfolate to bypass the bottleneck.

Particles flow at speed of pathway throughput.
Dietary folate leafy greens · folic acid DHF THF 5,10-methylene THF MTHFR + B2 cofactor normal L-5-Methyl-THF crosses BBB Methionine SAMe Homocysteine SAH B12 MTR/MTRR methylcobalamin Serotonin · DA · NE · Melatonin brain neurotransmitters BH4 (GCH1) cofactor Deplin / L-methylfolate 2.5–15 mg PO bypasses MTHFR 70% ↓
Clinical anchors:

Neurotransmitter Synthesis & Catabolism

Three parallel synthesis chains. Toggle a deficit to see what fails — and where. BH4 deficit kills serotonin AND dopamine. P5P (B6) deficit kills the final step of every line. Vitamin C deficit blocks NE synthesis (Catherine’s DBH problem). Inflammation diverts tryptophan into kynurenine — the toxin that gives you ‘flu brain.’

Particles flow at the speed of throughput.
SEROTONIN PATH Tryptophan (diet) TPH1/2 +BH4 +Iron +Vit D 5-HTP AADC +P5P Serotonin 5-HT Melatonin IL-6 → IDO ↑ Kynurenine neurotoxin · ‘flu brain’ CATECHOLAMINE PATH Tyrosine (diet) TH +BH4 +Iron L-DOPA AADC +P5P Dopamine DA DBH +Vit C +Cu NE norepinephrine Epi COMT clears → GABA PATH Glutamate (excitatory) GAD1 +P5P +Mg +Zn GABA (calming) → ↓ phobia · panic · anxiety
Serotonin
normal
Dopamine
normal
Norepinephrine
normal
GABA
normal
Kynurenine
trace
Clinical anchors:

CNR1 Tri-Loop — Catherine’s Pathology

When two aberrant CB1 receptors meet stress, three reinforcing loops crash the brain. Found in 3.2% of the population. The loops feed back on themselves — that’s why this profile is so devastating. Toggle Apply intervention to see PEA, olive oil, dark chocolate, and singing slow the cascade.

Watch the three loops accelerate, then break.
CNR1 2 aberrant copies 3.2% prevalence Acute stressor stress Cortisol / HPA Loop HPA axis activated ↑ Cortisol CB1 cannot brake HPA Amygdala primed for fear no brake Inflammation Loop Microglia activated ↑IL-1β ↑IL-6 ↑TNF-α ↓ GABA synthesis unrestrained Neurogenesis Loop ↓ CB1 ligands (AEA · 2-AG) ↓ BDNF ↓ Neuroplasticity ↓ Serotonin · emotional dysregulation no rescue PEA · EVOO · dark chocolate singing · running upregulates
Cortisol load
↑↑↑
Inflammation
↑↑↑
Neurogenesis
↓↓↓
CB1 signal
broken

Inflammation → BBB → Brain

Why every flu, every COVID, every infection moves your TRD patient backward. Toggle Insult to fire the cascade — TNF-α loosens BBB tight junctions, IL-6 enters and triggers the kynurenine shunt. Toggle NF-κB inhibitors (curcumin · CoQ10 · sulforaphane · omega-3 · PQQ) to seal the wall back up.

Watch the BBB tight junctions open and the kynurenine counter climb.
PERIPHERY · BLOOD BRAIN BBB tight junctions intact Insult virus · injury NF-κB master switch ON TNF-α IL-6 IL-1β IDO ↑ tryptophan shunt Kynurenine ‘cobweb-brain’ neurotoxin ↑ Cortisol → amygdala ↓ BDNF · neuroplasticity Mood ↓ · cognition ↓ · TRD setback Curcumin · CoQ10 · PQQ turns off
BBB integrity
intact
Brain cytokines
trace
Kynurenine load
trace
Cortisol
baseline
BDNF
normal

HPA Axis & Stress Response

The cortisol cascade. Click Stress! to fire it. Toggle Ashwagandha or Diaphragmatic breathing to dampen the response and watch the negative-feedback brakes engage.

Cortisol counter shows accumulation.
BRAIN Hypothalamus releases CRH CRH Pituitary releases ACTH ACTH Adrenal Gland releases Cortisol Cortisol Systemic Cortisol amygdala fear · ↓BDNF · ↓neuroplasticity Stressor tap "Stress!" Endocannabinoids · CB1 primary HPA brake brake ↓ Hippocampus negative feedback to HPA cortisol negative feedback Ashwagandha 500 mg BID ↓ adrenal cortisol output dampens Diaphragmatic breathing ↓ hypothalamic CRH
CRH
baseline
ACTH
baseline
Cortisol
baseline
Brake function
intact

Blood-Brain Barrier — Why Blood Tests Fail in Psych

Reed’s ‘Genetic Jobs’ Framework

Patients are not broken — they were optimized for a different environment. Each genotype maps to a behavioral archetype that helped our ancestors survive.

Hausman-Cohen’s ‘Fundamentals’ Pillars

Always check these four pillars before chasing diagnostic-specific genes — they apply to every chronic disease state. ‘Put out the fire first before remodeling the house.’ — Dr. Heather Way

Reed’s Treatment Algorithm

    Drug hierarchy

      Kraker’s Brain ‘Office’

      Memorize the personalities — they’re what fail in TRD.